Search results for "Potassium Compounds"

showing 9 items of 9 documents

Intracellular fluoride influences TASK mediated currents in human T cells.

2019

The expression of Kv1.3 and KCa channels in human T cells is essential for maintaining cell activation, proliferation and migration during an inflammatory response. Recently, an additional residual current, sensitive to anandamide and A293, compounds specifically inhibiting currents mediated by TASK channels, was observed after complete pharmacological blockade of Kv1.3 and KCa channels. This finding was not consistently observed throughout different studies and, an in-depth review of the different recording conditions used for the electrophysiological analysis of K+ currents in T cells revealed fluoride as major anionic component of the pipette intracellular solutions in the initial studie…

0301 basic medicinePatch-Clamp TechniquesTime FactorsPotassium CompoundsT-LymphocytesImmunologyMagnesium ChlorideMembrane Potentials03 medical and health scienceschemistry.chemical_compoundFluorides0302 clinical medicinePotassium Channels Tandem Pore DomainPotassium Channel BlockersImmunology and AllergyHumansCells CulturedKv1.3 Potassium ChannelActivator (genetics)ChemistryPipetteAnandamideElectrophysiology030104 developmental biologyMembraneBiophysicsCell activationFluorideIntracellular030215 immunologyJournal of immunological methods
researchProduct

Aequorin-expressing yeast emits light under electric control

2011

In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.

Activated proteinsLightScyphozoaAequorinApplied Microbiology and BiotechnologyElectricityHydroxidesCellular behaviorsPriority journalYeast electro-stimulationbiologyPhosphorescencePower generatorsGeneral MedicineElectrostimulationMAQUINAS Y MOTORES TERMICOSElectrodeElectric pulseInterphaseBioluminescenceYeast cultureGenetic EngineeringMATEMATICA APLICADABiotechnologyPotassium CompoundsLight pulse generatorsSaccharomyces cerevisiaeBioengineeringNanotechnologyLight pulseSaccharomyces cerevisiaeElectrical stimulationsYeast strainArticleAequorinBioluminescenceBiologySynthetic biologyBioelectronicsBioelectronicsbiology.organism_classificationElectric controlYeastElectric StimulationYeastFISICA APLICADABiophysicsbiology.proteinProtein expressionJellyfishElectrical controlLuminescenceCell functionTransgenics
researchProduct

Improving sensitivity in simultaneous determination of copper carboxylates by nonaqueous capillary electrophoresis

2005

Abstract A new method of nonaqueous capillary electrophoresis (NACE) with UV spectrophotometric detection was developed and optimized for the simultaneous determination of seven carboxylates (trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA), diethylenetriaminepentaacetic acid (DTPA), ethylenediaminetetraacetic acid (EDTA), N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA), nitrilotriacetic acid (NTA), 1,3-diaminopropane-N,N,N′,N′-tetraacetic acid (PDTA) and triethylenetetraaminehexaacetic acid (TTHA)) as copper complexes. The method development was carried out by using a fused silica capillary. Background electrolyte (BGE) was optimized and the best separation a…

BromidesAnalytePotassium CompoundsCarboxylic Acidschemistry.chemical_elementEthylenediaminetetraacetic acidBuffersSensitivity and SpecificityBiochemistryAnalytical ChemistryElectrolyteschemistry.chemical_compoundCapillary electrophoresisOrganometallic CompoundsChelationCarboxylateEdetic AcidChelating AgentsChromatographyFormamidesChemistryPotassium bromideOrganic ChemistryNitrilotriacetic acidElectrophoresis CapillaryGeneral MedicineHydrogen-Ion ConcentrationCopperCalibrationCopperWater Pollutants ChemicalJournal of Chromatography A
researchProduct

Panniculitis due to potassium bromide.

1998

Potassium bromide again is well known to be surprisingly effective in patients with severe myoclonic epilepsy in infants (SME). Rare side effects on the skin reappeared, such as the febrile nodular panniculitis (Weber-Christian syndrome). In 1993 we described the first three cases of necrotizing panniculitis and introduced the term 'halogen panniculitis'. It is a systemic disease with crops of subcutaneous nodules, fever, elevated sedimentation rate, hepatosplenomegalia, and abdominal pain. Later severe necrosis of the skin and adipose tissue may happen with deep ulcerations. History and course of five cases, described in this paper, suggest either an allergy or toxic reason. Histologic pic…

BromidesMaleSystemic diseaseAbdominal painPathologymedicine.medical_specialtyAllergyNecrosisPanniculitisAdolescentPotassium CompoundsAdipose tissueInflammationSkin DiseasesDevelopmental NeuroscienceRecurrenceSkin UlcermedicineHumansChildRetrospective StudiesEpilepsybusiness.industryGeneral Medicinemedicine.diseaseChild PreschoolPediatrics Perinatology and Child HealthMyoclonic epilepsyAnticonvulsantsFemaleNeurology (clinical)medicine.symptomPanniculitisbusinessBraindevelopment
researchProduct

Acetazolamide-M(II) [M(II) = Co(II), Ni(II), Cu(II)] complexes with ethylamine, diethylamine, triethylamine, and potassium hidroxide

1991

Abstract Complexes of Co(II), Ni(II), and Cu(II) with dideprotonated Acm are synthesized and characterized. Acm acts as bidentate ligand through the N-sulfonamido atom and the N-thiadiazole atom except for K6CoAcm4·6H2O in which Acm behaves as monodentate through the N-sulfonamido atom.

DenticityDiethylaminesPotassium CompoundsPotassiumInorganic chemistrychemistry.chemical_elementLigandsBiochemistryMedicinal chemistryInorganic Chemistrychemistry.chemical_compoundNickelEthylaminesHydroxidesmedicineTriethylamineDiethylamineChemistrySpectrophotometry AtomicCobaltAcetazolamideSpectrophotometryPotassiumEthylamineAcetazolamideCoppermedicine.drugJournal of Inorganic Biochemistry
researchProduct

The importance of potassium citrate and potassium bicarbonate in the treatment of uric acid renal stones

2017

Uric acid calculi can also be treated without surgery, with simple medical lytic therapy. After appropriate dietary adjustments and add of mineral water, the needed amount of alkali supplementation can increase pH values of the urine in order to dissolve the stones. Treatment should be prolonged to prevent stone recurrence. A case of bilateral renal uric acid stones that were successfully treated by alakalizing treatment was presented.

Malemedicine.medical_specialtyIncrease phPotassium CompoundsUrologyPotassiumUrologyUric acid stoneschemistry.chemical_elementUrineurologic and male genital diseaseslcsh:RC870-923Potassium bicarbonateKidney Calculichemistry.chemical_compoundmedicineHumansbusiness.industryfood and beveragesMiddle Agedlcsh:Diseases of the genitourinary system. UrologyPotassium citrateUric AcidBicarbonatesUric acid stoneschemistryUric acidbusinessArchivio Italiano di Urologia e Andrologia
researchProduct

Fluoride-induced precipitates on enamel surface and subsurface areas visualised by electron microscopy and confocal laser scanning microscopy

1997

The present study examined the enamel surface after in vitro topical treatments with a neutral 2% NaF solution. For minimising the risk of artefacts, samples were inspected without pre-treatment as fresh, naturally wet specimens by complementary techniques: variable pressure electron microscopy (VP-SEM) and confocal laser scanning microscopy (CLSM). VP-SEM provided information on the surface morphology, whereas CLSM allowed non-destructive visualisation of subsurface areas. Neutral NaF solutions induced globular precipitates on the enamel surfaces. If the globules formed may be described as "calcium fluoride like material", the additional information of this experiment is that, after intera…

Materials scienceMorphology (linguistics)AdolescentCausticsPotassium CompoundsConfocalAnalytical chemistrychemistry.chemical_elementlaw.inventionchemistry.chemical_compoundlawSodium fluorideHydroxidesChemical PrecipitationHumansFluorides TopicalChildDental EnamelTooth DemineralizationGeneral DentistryMicroscopy ConfocalEnamel paintSodiumtechnology industry and agricultureFluorineCariostatic Agentsbody regionsCalcium Fluoridechemistryvisual_artMicroscopy Electron ScanningFluorinevisual_art.visual_art_mediumSodium FluorideCalciumCrystalliteElectron microscopeArtifactsCrystallizationFluorideElectron Probe MicroanalysisEuropean Journal of Oral Sciences
researchProduct

Determination of total ribonucleotide pool in plant materials by high-pH anion-exchange high-performance liquid chromatography following extraction w…

2005

A new, improved method that only requires a potassium hydroxide extraction procedure is presented for the analysis of a full nucleotide pool in plant materials. Quantification was performed by high-pH anion-exchange chromatography (HPAEC) with UV detection after a potassium hydroxide extraction, and allowed the quantification of 13 linear ribonucleotides in a single run. The method has been validated by comparison of six extraction methods and also by measurement of the intracellular nucleotide levels of three plant species (cell cultures and leaves). The evolution of the nucleotide pool of Nicotiana tabacum cell culture during growth has also been measured, and showed an increase in the po…

RibonucleotidePotassium CompoundsNicotiana tabacumIon chromatographyBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundSpecies SpecificityHydroxidesSample preparationAnion Exchange ResinsPotassium hydroxideChromatographybiologyIon exchangeOrganic ChemistryExtraction (chemistry)General MedicineHydrogen-Ion ConcentrationPlantsReference StandardsRibonucleotidesChromatography Ion Exchangebiology.organism_classificationchemistryJournal of Chromatography A
researchProduct

Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil-miscible KOH/propanol/me…

2005

The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3-tert-butyl-4-hydroxyanisol and 2,6-di-tert-butyl-4-hydroxytoluene), E-vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil-miscible background electrolyte (BGE) was investigated. The BGE contained 40 mM KOH in a methanol/1-propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well-resolved among them and from the triacylglycerols. Using virgin palm, extra virgin oliv…

food.ingredientChromatographyCapillary actionPotassium CompoundsMethanolClinical Biochemistryfood and beveragesElectrophoresis CapillaryWheat germElectrolyte1-PropanolBiochemistrySoybean oilAnalytical ChemistryPropanolchemistry.chemical_compoundCapillary electrophoresisfoodchemistryPhenolsSolubilityYield (chemistry)HydroxidesPlant OilsMethanolElectrophoresis
researchProduct